Fig. 3

Representative images of AngioTool analysis and average vessel length determination. a Representative images (scale bars = 250 μm) of i single cells and ii developed vessel-network embedded within scaffolds, as captured through a confocal microscope. b The AngioTool program marks the i single cells (red) or ii developed vessels (red), junctions (blue) and vessel borders (yellow). c Average vessel length for different cell combinations was then determined. Two-way ANOVA, p value = 0.016, n ≥ 3. *p < 0.05, ***p < 0.001. HAMEC Human adipose microvascular endothelial cell, HNDF Human neonatal dermal fibroblast, HUVEC Human umbilical vein endothelial cell, MSC Mesenchymal stem cell