Fig. 3From: Long noncoding RNA Braveheart promotes cardiogenic differentiation of mesenchymal stem cells in vitroCardiogenic differentiation in the different cell groups. Cardiogenic differentiation of MSCs in the different cell groups was evaluated by the expressions of cTnT and α-SA. A Confocal microscopy of immunofluorescent staining of DAPI-labeled MSCs induced by 5-AZA after 14 days (200×). Cells stained with antibody to cTnT appeared green, and cells stained with antibody to α-SA appeared red. A1–A3 represented the expressions of cTnT and α-SA under normoxic condition, and A4–A6 represented the expressions of cTnT and α-SA under the hypoxia/reoxygenation (HR) condition; A1 and A4, A2 and A5, and A3 and A6 represented the blank control group, null vector control group, and lncRNA-Bvht group respectively. a Cells derived from DAPI-labeled MSCs induced by 5-AZA displayed blue nuclei; (b) Cells stained with antibody to cTnT appeared green in A1-A6; (c) Cells stained with antibody to α-SA appeared red in A1-A6; (d) Merged image of a, b, and c. B Comparison of the percentage of cTnT-positive cells among the different groups under normoxic condition and after hypoxia/reoxygenation respectively. C Western blot (a) and quantitative real-time PCR (b) analysis of the expressions of cTnT and α-SA in the different cell groups. **P <0.01 , versus blank control; ## P <0.01 , versus null vector controlBack to article page