Fig. 4

Human umbilical cord-derived mesenchymal stem cell exosomes (hucMSC-Ex) prevent cisplatin-induced apoptosis and the inflammatory response by activating autophagy in vitro. a The expression of LC3B, BCL-XL, BCL-2, Bax, and GAPDH proteins was measured by Western blot. b The expression of NF-κB-P65, histone, IL1-β, and GAPDH proteins was measured by Western blot. c ELISA analyses of tumor necrosis factor alpha (TNF-α) expression in NRK-52E cells. *P < 0.05, n = 3. NRK-52E cells treated as mentioned for the in vitro experiments were divided into six groups as follows: 1) control; 2) phosphate-buffered saline (PBS); 3) hucMSC-Ex; 4) hucMSC-Ex + 3-methyladenine (3MA); 5) 3MA; and 6) rapamycin (Rapa)