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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Bone regeneration by human dental pulp stem cells using a helioxanthin derivative and cell-sheet technology

Fig. 3

Osteogenic differentiation of DPSCs with TH in short-term culture. a DPSCs cultured in OM with or without TH (10–6 M). After 7 or 14 days, cells were subjected to ALP staining to detect ALP activity (n = 4). b RT-PCR performed to measure expression levels of osteogenic differentiation markers in DPSCs cultured with or without TH at day 7 or day 14. Gene expression levels of each sample plotted in the left graph, and the right graph shows the means of four samples. Error bars represent SD. Statistical significance determined by paired Student t test (*p < 0.05). d day, OM osteogenic medium, TH 4-(4-methoxyphenyl)pyrido[40,30:4,5]thieno[2,3-b]pyridine-2-carboxamide

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