Fig. 4
From: Differentiation of human induced pluripotent stem cells into nucleus pulposus-like cells
Images of hiPSC cultured for 36 days with nucleus pulposus differentiation media (NPDM) or basal media. a H&E staining of pellets cultured in NPDM (scale bar = 400 μM) or (b) basal medium showing formation of larger pellets with a vacuolated morphology when hiPSCs were promoted to differentiation in NPDM (scale bar = 400 μM). (c) When cultured in NPDM, human iPSCs contained vacuole-like structures (scale bar = 50 μm). d NPDM promoted expression of CD24, LM-α5, and BASP1 protein by 28 days of culture (scale bar = 50 μm). LMα5 alpha-5 subunit of heterotrimeric laminin, BASP1 brain abundant membrane attached signal protein 1