Fig. 2

ALA promotes early erythroid differentiation of CMPs. A Cytometry flow assays of CD123^– GPA^– CD34⁺ CD36^– BFU cells and CD123^– GPA^– CD34^– CD36⁺ CFU cells derived from CMPs were performed on day 4 in the presence or absence of ALA. B Cytometry flow assay of CD71⁺ GPA^– progenitors and CD71⁺GPA⁺ erythrocytes derived from CMPs were performed on day 12 in the presence or absence of ALA. C Statistical analysis of the percentages of CD71⁺ GPA^– progenitors and CD71⁺ GPA⁺ erythrocytes in the presence and absence of ALA were performed with data collected on day 12. The data in the bar graphs in panel (C) are presented as the mean ± SD. An unpaired Student’s t-test (2-tailed) was performed. N = 3–5 replicates; *P < 0.05, **P < 0.01